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FungiFlex® Mold Plus

Combined plasma cfDNA detection of invasive mold pathogens and P. jirovecii in a single panel.

Sample type
Plasma
Turnaround
<24h
after sample receipt
Test Code
FMPJ
CPT Code
87798

Results Reported

Everything in the Mold Panel, plus Pneumocystis jirovecii.

Organism detected Included in those organisms
Aspergillus flavusA. flavus
Aspergillus fumigatusA. fumigatus
Aspergillus nigerA. niger
Aspergillus terreusA. terreus
Fusarium spp. F. verticillioides, F. oxysporum, F. solani
Scedosporium Scedosporium spp., Lomentospora prolificans
Mucorales agentsMucor circinelloides, Rhizomucor miehei, Rhizopus microsporus, Rhizopus oryzae, Cunninghamella bertholletiae
Pneumocystis jiroveciiP. jirovecii

Clinical Utility

A single plasma cfDNA test for both invasive molds and Pneumocystis jirovecii — one blood draw.

Immunocompromised patients with suspected opportunistic fungal pneumonia are often evaluated for both invasive mold infections and Pneumocystis jirovecii pneumonia (PCP). Although these infections differ in their microbiology and management, they frequently occur in similar high-risk patient populations and may present with overlapping clinical features, making rapid diagnosis challenging. The FungiFlex® Mold Plus assay combines targeted plasma cell-free DNA (cfDNA) detection of invasive molds and Pneumocystis jirovecii in a single blood test, providing a comprehensive, targeted approach to support the evaluation of patients with suspected opportunistic fungal infection.

Specimen Instructions

Sample requirements

Container type
K2-EDTA (Lavender Top)
Sample volume
>4 mL Plasma or >8 mL Whole Blood
Preferred specimen
  • Whole Blood If received by the Zepto laboratory < 24 hours after collection
  • Plasma If received by the Zepto laboratory > 24 hours after collection
Sample Sendout Instructions (PDF)

Sample stability

Sample type Temperature Stability
Whole Blood Ambient Up to 24 hours
Plasma Ambient Up to 96 hours
Frozen Plasma ≤ −20°C Up to 4 weeks

Storage & shipping

  • If shipment will be delayed beyond 96 hours, freeze plasma at ≤ −20°C and ship on dry ice.

  • All specimens must be accompanied by a Zepto Test Requisition form and labeled with the patient's name, another unique identifier, and collection time.

  • Specimens must be triple packaged according to IATA Instruction 650. Ship the specimen using UPS Next Day Air Early or FedEx First Overnight.

  • Frozen specimens must be shipped on dry ice inside an appropriate insulated shipping container.

How to order this test

Forms & More Information

NOT FDA-CLEARED · THIS TEST WAS DEVELOPED AND ITS PERFORMANCE CHARACTERISTICS ESTABLISHED BY ZEPTO LIFE TECHNOLOGY, IN A MANNER CONSISTENT WITH CLIA REQUIREMENTS.

References

01Vergidis, P. et al. A Novel Giant Magnetoresistance—Enabled Multiplex Polymerase Chain Reaction Assay for the Diagnosis of Invasive Fungal Infection Open Forum Infectious Diseases, 2025. academic.oup.com/ofid
02Vergidis, P. et al. Diagnosis of Invasive Fungal Infection Using a Cell-Free DNA Assay Coupled with GMR Lab-on-a-Chip Technology. Presented at ECCMID, 2024.
03Vergidis, P. et al. Detection of Aspergillus fumigatus Cell-Free DNA Using Giant Magnetoresistance Technology. Presented at Advances Against Aspergillosis, 2024.