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FungiFlex®
Pneumocystis jirovecii

Targeted plasma cfDNA detection of P. jirovecii.

Sample type
Plasma
Turnaround
<24h
after sample receipt
Test Code
FPJ01
CPT Code
87798

Result Reported

Organism detected Included in those organisms
Pneumocystis jiroveciiP. jirovecii

Clinical Utility

A plasma cfDNA assay offers a rapid, noninvasive way to evaluate suspected Pneumocystis pneumonia (PCP).

Pneumocystis pneumonia, caused by Pneumocystis jirovecii, is a serious opportunistic fungal pneumonia that primarily affects immunocompromised patients, including transplant recipients, patients with hematologic malignancies, individuals living with HIV, and those receiving immunosuppressive therapies. Early diagnosis is critical because untreated PCP can be life-threatening, yet diagnosis often relies on bronchoalveolar lavage (BAL), induced sputum, or nonspecific laboratory findings. The FungiFlex® Pneumocystis jirovecii assay uses plasma cell-free DNA (cfDNA) to provide a rapid, noninvasive approach for detecting Pneumocystis jirovecii from a simple blood sample, supporting the evaluation of patients with suspected PCP.

Specimen Instructions

Sample requirements

Container type
K2-EDTA (Lavender Top)
Sample volume
>2 mL Plasma or >4 mL Whole Blood
Preferred specimen
  • Whole Blood If received by the Zepto laboratory < 24 hours after collection
  • Plasma If received by the Zepto laboratory > 24 hours after collection
Sample Sendout Instructions (PDF)

Sample stability

Sample type Temperature Stability
Whole Blood Ambient Up to 24 hours
Plasma Ambient Up to 96 hours
Frozen Plasma ≤ −20°C Up to 4 weeks

Storage & shipping

  • If shipment will be delayed beyond 96 hours, freeze plasma at ≤ −20°C and ship on dry ice.

  • All specimens must be accompanied by a Zepto Test Requisition form and labeled with the patient's name, another unique identifier, and collection time.

  • Specimens must be triple packaged according to IATA Instruction 650. Ship the specimen using UPS Next Day Air Early or FedEx First Overnight.

  • Frozen specimens must be shipped on dry ice inside an appropriate insulated shipping container.

How to order this test

Forms & More Information

NOT FDA-CLEARED · THIS TEST WAS DEVELOPED AND ITS PERFORMANCE CHARACTERISTICS ESTABLISHED BY ZEPTO LIFE TECHNOLOGY, IN A MANNER CONSISTENT WITH CLIA REQUIREMENTS.

References

01Young, J.A.H. et al. Extrapulmonary and Other Pneumocystis jirovecii Infections Diagnosed via Fungal Cell-Free DNA Using Giant Magnetoresistance Detection. Journal of Fungi, 2026. doi.org/10.3390/jof12060435
02Vergidis, P. et al. A Novel Giant Magnetoresistance—Enabled Multiplex Polymerase Chain Reaction Assay for the Diagnosis of Invasive Fungal Infection Open Forum Infectious Diseases, 2025. academic.oup.com/ofid